Ac on Chromosome 10
Chromosome 10 of maize showing the position of mapped Ac elements
Current Status (last updated 05/06/04). 2 mapped.
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10 Ac collection in any of the following ways:
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browse Ac elelments on chromosome 10. Ac listed by map position
north to south.
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Ac-bti99224
Typical kernel variegation patterns observed in seed homozygous for Ac-bti99224 and the Ds reporter (Ac-bti99224/Ac-bti99224, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-bti99224 was initially cloned from a ~2.0 kb band, isolated from a genomic digestion using EcoR1/Kpn1 and hybridized with a probe designed from the internal bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 10.01/10.02 (IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: AY559201
Ac-mon00218
The flanking sequence of Ac-mon00218 was initially cloned from a ~5.5 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 700 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 10.07 (IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: AY559185