Ac on Chromosome 3
Chromosome 3 of maize showing the position of mapped Ac elements
Current Status (last updated 02/14/05). 6 mapped.
A number of seed stocks are currently available through the Maize Genetics CoOp. Contact Tom Brutnell (tpb8@cornell.edu) for further details.
Search the chromosome 3 Ac collection in any of the following ways:
1) Scroll down to
browse Ac elelments on chromosome 3. Ac listed by map position
north to south.
2) Click on bin numbers
listed below the map to jump to the first Ac in that bin.
3) Use the jump menus
below the map to go direct to data on a named Ac.
The flanking sequence of Ac-bti03702 was initially cloned from a ~2.5 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 900 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 3.04 (IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: NA
Ac-bti00220
Typical kernel variegation patterns observed in seed homozygous for Ac-bti00220, and the Ds reporter (Ac-bti00220/Ac-bti00220, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-bti00220 was initially cloned from a ~4.7 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 700 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 3.04 (mapping data is currently unavailable)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: AY559218
Ac-mon00178
Typical kernel variegation patterns observed in seed homozygous for Ac-mon00178, and the Ds reporter (Ac-mon00178/Ac-mon00178, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-mon00178 was initially cloned from a ~3.4 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 900 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position : 3.05 (IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: AY559203
Ac-bti03526
The flanking sequence of Ac-bti03526 was initially cloned from a ~2.7 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 900 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 3.05/3.06 (IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: NA
Ac-bti31083
The flanking sequence of Ac-bti31083 was initially cloned from a ~2.8 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 700 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 3.06/3.07 (IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: NA
The flanking sequence of Ac-bti03616 was initially cloned from a ~3.0 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 700 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 3.09 (IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: NA