Ac on Chromosome 4
Chromosome 4 of maize showing the position of mapped Ac elements
Current Status (last updated 08/09/05). 9 mapped.
A number of seed stocks are currently available through the Maize Genetics CoOp. Contact Tom Brutnell (tpb8@cornell.edu) for further details.
Search the chromosome 4 Ac collection in any of the following ways:
1) Scroll down to
browse Ac elelments on chromosome 4. Ac listed by map position
north to south.
2) Click on bin numbers
listed below the map to jump to the first Ac in that bin.
3) Use the jump menus
below the map to go direct to data on a named Ac.
Ac-mon00126
Typical kernel variegation patterns observed in seed homozygous for Ac-mon00126, and the Ds reporter (Ac-mon00126/Ac-mon00126, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-mon00126 was initially cloned from a ~5.0 kb band, isolated from a genomic digestion using Pst1 and hybridized with a probe designed from the internal EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map
position: 4.02/4.03 (IBM population)
Map coordinate at MaizeGDB
Position in GSS Assembly
Seed in stock. Genbank Accession #: AY559211
Ac-bti04066
Our newest Ac line: more information will be here soon.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 4.05 (IBM population)
Seed is not currently available. Genbank Accession #: NA
Ac-mon03073
Typical kernel variegation patterns observed in seed homozygous for Ac-mon03073, and the Ds reporter (Ac-mon03073/Ac-mon03073, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-mon03073 was initially cloned from a ~3.4 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 900 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed
through PCR verification, as
well as through Southern blot verification.
We highly recommend confirming the position of all Ac lines using
both PCR and Southern blot verification prior to large scale mutagenesis
(see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 4.06
(IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: AY559213
Ac-bti00209
Typical kernel variegation patterns observed in seed homozygous for Ac-bti00209, and the Ds reporter (Ac-bti00209/Ac-bti00209, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-bti00209 was initially cloned from a ~2.7 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 900 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 4.06
(IBM population)
Map coordinate at MaizeGDB
Position in GSS Assembly
Seed in stock. Genbank Accession #: AY559195
Ac-bti00207
Typical kernel variegation patterns observed in seed homozygous for Ac-bti00207, and the Ds reporter (Ac-bti00207/Ac-bti00207, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-bti00207 was initially cloned from a ~4.6 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 900 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 4.06/4.07 (IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: AY618476
Ac-bti31094
The flanking sequence of Ac-bti31094 was initially cloned from a ~5.5 kb band, isolated from a genomic digestion using Pst1 and hybridized with a probe designed from an internal EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 4.07 (IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: NA
Ac-bti41832
Information coming soon!
Map position: 4.07/4.08 (IBM population)
Seed not currently available. Genbank Accession #: NA
Ac-bti00245
Typical kernel variegation patterns observed in seed homozygous for Ac-bti00245, and the Ds reporter (Ac-bti00245/Ac-bti00245, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-bti00245 was initially cloned from a ~3.1 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 700 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 4.08
(IBM population)
Map coordinate at MaizeGDB
Position in GSS Assembly
Seed in stock. Genbank Accession #: AY559181
Ac-bti31192
The flanking sequence of Ac-bti31192 was initially cloned from a ~3.0 kb band, isolated from a genomic digestion using Pst1 and hybridized with a probe designed from the internal 700 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 4.09 (IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: NA