Ac on Chromosome 5
Chromosome 5 of maize showing the position of mapped Ac elements
Current Status (last updated 05/04/04). 7 mapped.
A number of seed stocks are currently available through the Maize Genetics CoOp. Contact Tom Brutnell (tpb8@cornell.edu) for further details.
Search the chromosome
5 Ac collection in any of the following ways:
1) Scroll down to
browse Ac elelments on chromosome 5. Ac listed by map position
north to south.
2) Click on bin numbers
listed below the map to jump to the first Ac in that bin.
3) Use the jump menus
below the map to go direct to data on a named Ac.
Ac-mon00212
Typical kernel variegation patterns observed in seed homozygous for Ac-mon00212 and the Ds reporter (Ac-mon00212/Ac-mon00212, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-mon00212 was initially cloned from a ~5.4 kb band, isolated from a genomic digestion using Pst1 and hybridized with a probe designed from the internal EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 5.01 (IBM population)
Map coordinate at MaizeGDB
Position in GSS Assembly
Seed in stock. Genbank Accession #: AY559232
Ac-mon00030
Typical kernel variegation patterns observed in seed homozygous for Ac-mon00030 and the Ds reporter (Ac-mon00030/Ac-mon00030, r-sc:m3/r-sc:m3)
(photo currently unavailable)
The flanking sequence of Ac-mon00030 was initially cloned from a ~3.8 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 900 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 5.03/5.04 (IBM population)
Map coordinate at MaizeGDB
Position in GSS Assembly
Seed in stock. Genbank Accession #: AY559197
Ac-mon00152
Typical kernel variegation patterns observed in seed homozygous for Ac-mon00152, and the Ds reporter (Ac-mon00152/Ac-mon00152, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-mon00152 was initially cloned from a ~10.0 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 900 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 5.04/5.05 (IBM population)
Map coordinate at MaizeGDB
Position in GSS Assembly
Seed in stock. Genbank Accession #: AY559217
Ac-mon00044
Typical kernel variegation patterns observed in seed homozygous for Ac-mon00044, and the Ds reporter (Ac-mon00044/Ac-mon00044, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-mon00044 was initially cloned from a ~3.0 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 900 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 5.06 (IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: AY559219
Ac-mon00238
Typical kernel variegation patterns observed in seed homozygous for Ac-mon00238, and the Ds reporter (Ac-mon00238/Ac-mon00238, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-mon00238 was initially cloned from a ~5.2 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 900 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 5.06 (IBM population)
Map coordinate at MaizeGDB
Seed in stock. Genbank Accession #: AY559194
Ac-bti00194
Typical kernel variegation patterns observed in seed homozygous for Ac-bti00194, and the Ds reporter (Ac-bti00194/Ac-bti00194, r-sc:m3/r-sc:m3)
The flanking sequence of Ac-bti00194 was initially cloned from a ~2.8 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 900 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 5.07/5.08 (mapping data currently unavailable)
Map coordinate at MaizeGDB
Position in GSS Assembly
Seed in stock. Genbank Accession #: AY559209
Ac-mon03082
Typical kernel variegation patterns observed in seed homozygous for Ac-mon03082, and the Ds reporter (Ac-mon03082/Ac-mon03082, r-sc:m3/r-sc:m3)
(photo currently unavailable)
The flanking sequence of Ac-mon03082 was initially cloned from a ~4.0 kb band, isolated from a genomic digestion using EcoR1 and hybridized with a probe designed from the internal 700 bp EcoR1-HindIII fragment of Ac.
Insertion of the Ac element into the cloned sequence was confirmed through PCR verification, as well as through Southern blot verification. We highly recommend confirming the position of all Ac lines using both PCR and Southern blot verification prior to large scale mutagenesis (see Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.
Map position: 5.08/5.09 (BNL population)
Map coordinate at MaizeGDB
Position in GSS Assembly
Seed in stock. Genbank Accession #: AY559210