Ac-mon03073 PCR verification
Ac-mon03073
PCR verification gel:
Lane 1 = P-vv
Lane 2 = Ac-mon03073
Lane 3 = 100 bp ladder
Lane 4 = 100 bp ladder
Lane 5 = Ac-mon03073.1
Lane 6 = Ac-mon03073.2
Lane 7 = Ac-mon03073.4
Lane 8 = Ac-mon03073.5
Lane 9 = Ac-bti97156
PCR verification primers:
Ac (3073.9 Ac): 5’-TTTCGTTTCCGTCCCGCAAGTT-3’
flanking (mon03073.9F): 5’-GGAAGCCCCTACTAAGATTTT-3’
Product size: 450 bp
PCR verification notes:
The DNA flanking the newly transposed Ac was cloned and sequenced from
one individual line. Verification PCR was performed using lines (lanes 2, 5
to 8) that contained the newly transposed Ac element. An Ac-specific
primer was used with a gene-specific primer to amplify the fragment of 450 bp.
The absence of this Ac from the progenitor line results in the absence
of the PCR product (lane 1). Detailed descriptions of Ac verification can be
found in Brutnell and Conrad, 2003, Methods in Molecular Biology, 236:157-76.