Tomato Microarray

The array contains approximately 12,000 spots. In an effort to make these arrays useful for a wide range of gene expression research these unigenes have been selected at random from a number different cDNA libraries made from a range of tissue including leaf, root, fruit, and flowers.

As well as the random unigenes the arrays will contain both positive and negative control spots.

The gene fragments are printed in 32 sub-grids, in a 21 by 20 spot arrangement per sub-grid. Complete meta-grid size is 13,440 spots per array (including negatives). The array can be hybridized under a 20X50mm coverslip.

A complete gene id file (gene map) of the array will be supplied with all orders, as well as being posted as a downloadable file from the CGEP website.

The gene id file now uses a generic file listing array spot ids. To retrieve the SGN number as well as more information for a particular gene you can paste that identifier in the search field at the SGN site here at cornell:

SGN WEBSITE

CLICK HERE TO SEE THE LAYOUT OF THE ARRAY

• The entire set of fragments on this array has been re-sequenced to verify the identity of all the gene fragments. All re-sequence information is available at the SGN site.

• As is often seen with high-through-put EST sequencing data we have discovered that there may be approximately 15-20% mis-annotation of particular clones. We highly recommend that prior to functional study of a particular gene that researchers obtain the clone of interest from Clemson University and have its sequence verified. A link to the Clemson site where you can order clones is listed below:

• Spots on the array that have failed our quality control measures will be listed as such in the gene id file. Do not analyze data from these spots.

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