Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
Graduate student André Velásquez from Greg Martin’s lab shows how to do Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato.
Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
Research associate Suma Chakravarthy from the Greg Martin’s lab, shows the procedure Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants.
Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis
BTI scientists recently exhibited their acting skills for the Journal of Visualized Experiments (http://www.jove.com). This video demonstrate how to mate and separate Chlamydomonas reinhardtii tetrads for genetic analysis and also how bugs interact with host plants.
The video by Xingshan Jiang explains the protocol for mating and tetrad separation of Chlamydomonas reinhardtii for genetic analysis.
The White Cabbage Butterfly
The video by Martin de Vos explains how you can identify which plants are more resistant to tissue chewing caterpillars. He uses a no-choice set-up where caterpillars are forced to feed from a plant. After 7 days Martin compares the weight of the caterpillars. Some caterpillars will be full-grown, while others are still small. Small caterpillars mean that a plant is well defended against these attackers. The second experiment is a no choice experiment with white cabbage butterflies that are given a choice on which plant material to lay their eggs. The results show that these butterflies are attracted to plants with specific chemicals in the leaves.
How To Set Up Aphid Artificial Diet Experiments
The video made by John Ramsey shows how some specialized green peach aphids (Myzus persicae) are able to feed from tobacco, whereas others cannot. This is likely because of the presence of nicotine. Nicotine is a toxic chemical that is not only present in cigarettes, but also in tobacco plants. John uses an artificial diet experiment to show that nicotine does not affect red aphids, but kills green aphids that belong to the same species. John is now trying to identify why these red aphids are able to withstand higher concentrations of nicotine in their food.
Aphids That Transmit Plant Viruses
Cecilia also got her chance to shine in the spotlight of JoVE’s cameras. She studies aphids that transmit plant viruses and shows her viewers how to study virus transmission between infected plants, aphids, and non-infected plants. She uses a technique whereby aphids are injected with virus particles to determine their ability to transmit this virus.
DNA Sequencing Puzzle
The activity includes background information on tomatoes, DNA, and various molecular biology terms and techniques.
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BrachyBio! a groundbreaking new project getting kids excited about plant science!
Some of the most challenging societal problems on the planet will require plants to solve them; major shifts in agricultural land use due to global warming, pressures on food supplies due to growing populations and meeting energy demands with biofuels, to name a few. Dr. Tom Brutnell describes how BrachyBio! a groundbreaking new project is getting kids excited about plant science and the process of discovery while allowing them to contribute to scientific discovery in a meaningful way.
- BrachyBio! Interview (Air date 2010.11.23)
Interviews from the nationally syndicated series Sign of Science.
Tom Brutnell Interviewed by NPR
A study co-authored by Tom Brutnell has identified a gene in the carotenoid pathway that helps determine the levels of provitamin A in corn seeds.
- NPR Interview (Air date 2008.01.20)
Tracy Rosebrock Interviewed by Nature Magazine
Tracy Rosebrock, a BTI graduate student in the laboratory of Dr. Greg Martin, discusses a bacteria that can suppress plant immunity.
Welcome to BrachyBio! Linking Teachers, Students and Scientists in Plant Research
22 Hours of Light: Conditions for Optimal Growth
7 Day Cold Treatment: Preparing Brachypodium Seeds for Quick Growth in your Classroom
12 Seeds per Family: Planting Brachypodium Seeds