Tomato receptor FLAGELLIN-SENSING 3 activates plant immunity. Submitted. 2015Hind SR, Strickler SR, Boyle PC, Bao Z, O’Doherty IM, Baccile JA, Dunham DM, Viox EG, Clarke CR, Vinatzer BA, Schroeder FC and Martin GB.Submitted
Natural variation for responsiveness to flg22, flgII-28, and csp22 and Pseudomonas syringae pv. tomato in heirloom tomatoes 2014Veluchamy S, Hind SR, Dunham DM, Martin GB and Panthee DR.PLoS One 9(9), e106119
Allelic variation in two distinct Pseudomonas syringae flagellin epitopes modulates the strength of plant immune responses but not bacterial motility 2013Clarke CR, Chinchilla D, Hind SR, Taguchi F, Miki R, Ichinose Y, Martin GB, Leman S, Felix G and Vinatzer BA.New Phytologist 200(3), 847-860
The COP9 signalosome controls jasmonic acid synthesis and plant responses to herbivory and pathogens 2011Hind SR, Pulliam S, Veronese P, Shantharaj D, Nazir A, Jacobs N, and Stratmann JW.The Plant Journal 65(3), 480-491
Wounding systemically activates a mitogen-activated protein kinase in forage and turf grasses 2011Dombrowski JE, Hind SR, Martin RC, and Stratmann JW.Plant Science 180(5), 686-693
Gene silencing goes viral and uncovers the private life of plants 2011Stratmann JW and Hind SR.Entomologia Experimentalis et Applicata 140, 91-102
Tissue-type specific systemin perception and the elusive systemin receptor 2010Hind S, Malinowski R, Yalamanchili R, and Stratmann JW.Plant Signaling and Behavior 5(1), 42-44
Systemin and jasmonic acid regulate constitutive and herbivore-induced systemic volatile emissions in tomato, Solanum lycopersicum 2010Degenhardt DC, Hind SR, Stratmann JW, and Lincoln DE.Phytochemistry 71(17-18), 2024-2037
The goal of my research is to improve upon the natural resistance of crop plants in order to reduce our dependency on pesticides and to improve crop yields, thereby contributing to sustainable agricultural systems and to feeding the growing population in the world. My future research will focus on the perception of microbial and herbivore-associated signals by the plant defense machinery. I will expand on my experience working with tomato, insect pests and microbes, combined with methods from molecular biology, genetics, and biochemistry in order to increase our understanding about the molecular players involved in plant defense systems.
Current Research Projects
The focus of my research in the Martin Lab is the identification of plant proteins involved in the perception of microbial pathogens of tomato. Previous work I contributed to showed that, in addition to the well-studied flg22, a second conserved region of bacterial flagellin, called flgII-28, is recognized by tomato in an FLS2-independent manner (Clarke et al., 2013). Additionally, I worked with a tomato breeding program at North Carolina State University to screen heirloom tomato varieties in order to evaluate their differential responses to treatment with different microbe-associated signals, including flgII-28 (Veluchamy et al., 2014). Using an flgII-28 insensitive cultivar identified in this screen, I isolated the flgII28 receptor, called FLS3, by bulked segregant analysis and a mapping-by-sequencing approach (Hind et al. submitted). I confirmed direct binding of flgII-28 to FLS3 using a novel photo-affinity labeling strategy developed in collaboration with the Schroeder lab and funded by the TRIAD Foundation. I also demonstrated that bacterial effector proteins could suppress FLS3 signaling. I am currently exploring sequence variants of both FLS3 and flgII-28 found in natural populations which I anticipate, when combined with structural information provided by a collaborating laboratory, will illuminate key regions essential for FLS3 binding to flgII-28. This information may assist in targeted engineering of FLS3 to broaden the potential for recognition of a diverse group of bacterial pathogens.