User Manuals

Leica SP5 Confocal Microscope:

The updated confocal manual version is available here. This manual is for the use of trained people only. If you never used our confocal, you must contact PCIC to schedule a Training.

Olympus Stereomicroscope:

More information is provided in the facility.

Leica Confocal SP5 Application Notes:

Please find below a list of specific applications available with our confocal system. We can help you adjust your parameters contact PCIC.

How To

Choose From The Following Links

• Limit the crosstalk between your fluorophores
• Zoom in the live window and other usefull live tools
• Draw a Region Of Interest
• Scan only a Region Of Interest
• Perform a lamda scan
• Perform a sequential scan
• Enhance very weak signals
• Compensate for the loss of intensity along the z axis during an XYZ stack
• Export data and reports
• Perform a Time-Lapse experiment
• Use the FRET SE or FRET photobleaching wizards. If you never performed FRET, please contact PCIC