The Boyce Thompson Institute (BTI) Center for Plant Biotechnology Research was established to support projects at BTI that utilize genetic engineering and plant cell culture. The center is set up to provide plant transformations on a fee-based service for all BTI researchers. Center personnel are experienced at Agrobacterium tumefaciens–mediated transformation of tomato, potato, Nicotiana tabacum, and Nicotiana benthamiana. In addition to these systems, we are currently becoming familiar with methodology for Agrobacterium tumefaciens–mediated transformation of Medicago truncatula and Brachypodium distachyon.
If you are interested in obtaining information related to plant transformation experiment requests, contact Joyce Van Eck. If you decide to proceed with a request, follow the instructions below to access the online request system.
Complete one transformation request form for each construct. The online request form can be accessed at https://btiscience.samanage.com/. For BTI staff, use your BTI username and password. For groups outside of BTI, contact our Information Technology Administrator, Brian Klotz (firstname.lastname@example.org). Let him know that you need access to complete a transformation request. You will need to provide your e-mail address and a password to Brian. Once you have access, the form can be accessed after you login.
We are also available for instruction on transformation methods and for development of methodologies for plant species other than those currently being done in the center. The center is open to postdoctoral and research associates, graduate students, visiting scientists, and research assistants who are interested in using the facility to do their own plant cell/tissue culture and plant transformations.