The Boyce Thompson Institute Mass Spectrometry Center (BMSC) provides mass spectrometry services to BTI and Cornell University. The instrumentation in the facility is optimized for the analysis of small molecules, facilitating identification of known and unknown metabolites as well as comprehensive metabolic profiling. The facility aims to enable biologists of all levels of expertise to perform mass spectrometric small molecule analyses.
The facility is equipped with a Thermo Q-Exactive Orbitrap coupled to Dionex ultra-high pressure chromatography systems, a Micromass Quattro II triple-quadrupole spectrometer with MS/MS capabilities, a Varian 1200L GC-MS, and a Micromass Platform LC Mass Spectrometer enabling HPLC-MS, HPLC-MS/MS, and GC/MS analyses for structural and biological characterization of metabolites.
Training and assistance with the use of the mass spectrometers, software, and data analysis are provided. In addition, the BMSC offers mass spectrometric analyses on a fee-for-service basis.
The Thermo Q-Exactive Orbitrap coupled to Dionex ultra-high-pressure chromatography systems is capable of ionization using heated electrospray (HESI), atmospheric pressure chemical ionization (APCI), and nano-flow electrospray (Nano-ESI). The Orbitrap Mass Analyzer combined with advanced quadrupole, S-lens, and a high-performance HCD collision cell for MS/MS plus features such as 12 hz scan rate, data-independent analysis (DIA), selected-ion monitoring (SIM), and spectral multiplexing up to ten precursors/scan make this system a state-of-the-art instrument for chemical and biochemical molecules analysis with 140,000 resolution, < 1 ppm mass accuracy, and femtogram sensitivity.
The Dionex ultra-high-pressure liquid chromatography systems coupled with multiple switch valves and UV data acquisition makes it the system of choice for fully automated high-throughput approaches in the fields of metabolomics, proteomics, and food and environmental safety.
Varian 1200L triple quadrupole MS connected to a Varian CP3800 GC equipped with CombiPal autosampler, with SPME (solid phase micro-extraction) capability, and flame ionization detector.
Micromass Quattro II triple quadrupole MS connected to a Agilent 1100 series HPLC with glass vial autosampler and diode array detector.
Waters 2790 HPLC
- Waters 2475 Multi Fluorescence Detector
- Waters 2487 Dual Absorbance Detector
Waters 2695 HPLC
- Waters 2996 Photodiode Array Detector
- Waters 2700 Sample Manager
Training is available to all researchers at BTI and Cornell University. Users with previous mass spectrometry and/or GC/HPLC experience are required to take at least four hours of training. Additional training blocks are available for less experienced users. Training includes method development specifically tailored for each biological application.
Currently, the mass spectrometer is in high demand. To find out about future availability, please contact Navid Movahed.
Recommended plant-related general metabolomics and mass spectrometry literatue:
Plant Metabolomics (Biotechnology in Agriculture and Forestry) Kazuki Saito, Richard A. Dixon , Lothar Willmitzer
|Concepts in Plant Metabolomics B.J. Nikolau, Eve Syrkin Wurtel|
Metabolome Analysis: An Introduction (Wiley – Interscience Series on Mass Spectrometry) Silas G. Villas-Boas , Jens Nielsen, Jorn Smedsgaard, Michael A. E. Hansen , Ute Roessner-Tunali
|Mass Spectrometry: A Textbook Jürgen H. Gross|
Towards the Plant Metabolome and Beyond by Robert L. Last, A. Daniel Jones, and Yair Shachar-Hill, Nature Reviews Molecular Cell Biology 8 (2007): 167–174
Metabolomics–The Link between Genotypes and Phenotypes by Oliver Fiehn, Plant Molecular Biology 48(1–2) (2002): 155–171
Mass Spectrometry in Metabolome Analysis by Villas-Bôas, S. G., Mas, S., Åkesson, M., Smedsgaard, and J. Nielsen, Journal of Mass Spectrometry Reviews 24 (2005): 613–646
The facility computers are available for off-line processing and analysis of MS data acquired in the facility. Software includes Waters MassLynx and Varian MS Workstation.
To schedule an appointment for training or for questions, please contact Navid Movahed.
Our mailing address:
Manager, Mass Spectrometer Facility
Office: Room 409
Boyce Thompson Institute for Plant Research
533 Tower Road
Ithaca, NY 14853-1801